Protein Kinases are a family of pivotal enzymes implicated in various cell signaling pathways, constituting over 2% of the human genome. Kinases exert their roles by phosphorylating their respective physiological substrates. They catalyze the transfer of γ-phosphate from ATP or GTP to the hydroxyl groups of serine, threonine and tyrosine residues on the substrate proteins. Deregulation of Kinase could lead to the changes in enzymatic activity and/or expression, subcellular localization, the stability of kinase, protein-protein interactions. Aberrant regulation of Kinase-mediated signaling pathways is responsible for all major human pathological conditions such as cancer, cardiovascular disease, neurodegenerative diseases and metabolic disorders. ICE have more than 400 kinase assay including mutants ready to use. The assay methods are TR-FRET or ADP-Glo.