In the rapidly evolving field of biomedical research, understanding cell behavior under various conditions is critical for drug discovery, toxicity testing, and cancer research. 2D Cell viability, cell proliferation, and colony formation assays are essential tools for evaluating the effects of compounds on cellular health, growth, and survival. These assays provide invaluable insights into cellular responses, aiding researchers in making informed decisions throughout the drug development process. For those interested in regular tumor cell panel screening, we recommend our ICECP™ cancer cell panel screening service, which is specifically designed to handle large-scale tumor cell screening with precision and efficiency.
Our Cell Viability, Cell Proliferation, and Colony Formation Assays are designed to meet the diverse needs of researchers across multiple disciplines:
✅ Drug Discovery: Evaluate the cytotoxic effects of new chemical entities (NCEs) or biologics on various cell types, including tumor cells, gene-edited cells.
✅ Toxicity Testing: Determine the safety profile of compounds by assessing their impact on cell viability and proliferation.
✅ Cancer Research: Investigate the efficacy of anti-cancer agents through detailed analysis of cell proliferation and colony formation.
✅ Mechanistic Studies: Understand cellular mechanisms by analyzing cell cycle progression, DNA synthesis, and long-term survival in response to therapeutic interventions.
1. Cell Viability Assays
We offer a range of cell viability assays to suit your research needs, utilizing the following methods:
CellTiter-Glo Assay: Measures ATP levels as an indicator of metabolically active cells.
CellTiter-Blue Assay: Uses resazurin reduction to measure the number of viable cells.
MTT Assay: Detects living cells based on their ability to reduce MTT to formazan.
CCK-8 Assay: Provides a colorimetric assessment of cell viability via WST-8.
Nuclear Stain Assays: Utilizes fluorescent dyes to distinguish between live and dead cells.
2. Cell Proliferation Assays
Our cell proliferation assays are designed to quantify the growth rate of cells over time:
CyQUANT Assay: Measures cellular DNA content as an indicator of cell proliferation.
BrdU Incorporation Assay: Detects newly synthesized DNA in proliferating cells, providing a precise measure of cell proliferation.
3. Colony Formation Assays
The colony formation assay is a powerful technique to assess the long-term proliferative potential of cells:
Crystal Violet Staining: Visualizes and quantifies colonies formed by individual cells, allowing for the assessment of clonogenic survival and proliferative capacity.
Assay Formats
Our assays are highly flexible and can be tailored to your experimental requirements:
▶ 2D Assay Format: Conducted in 96-well or 384-well plates, suitable for high-throughput screening. These assays can be designed for short-term (3 to 6 days) or long-term (7 to 14 days) proliferation studies.
▶ Clonogenic Assay: Performed in 24-well, 12-well, or 6-well plates, allowing for the detailed analysis of colony formation over 10 to 14 days.
▶ Comprehensive Analysis: Our suite of assays provides detailed insights into cell viability, proliferation, and survival, crucial for evaluating therapeutic efficacy and toxicity.
▶ High Throughput: With the capability to perform assays in 96-well and 384-well formats, we offer scalable solutions for both small and large compound libraries.
▶ Customization: We tailor our assays to meet the specific needs of your research, whether you require single-agent testing or combination studies.
Our Cell Viability, Cell Proliferation, and Colony Formation Assays are robust, reliable, and designed to deliver high-quality data to support your research objectives. Whether you are involved in drug development, toxicity testing, or cancer research, our assays provide the precision and flexibility needed to advance your scientific endeavors.
We value your inquiries and are here to provide you with tailored solutions for your drug discovery and development needs. Whether you have questions, require more information, or are interested in discussing potential collaborations, our team of experts is just a message away.
Feel free to reach out to us.
