The NanoBRET Protein-Protein Interaction (PPI) Assay is a live-cell, real-time method for studying protein-protein interactions and evaluating how small molecules or biological factors affect these interactions. Using NanoLuc luciferase and HaloTag ligand (HL), the assay enables sensitive detection of both PPI induction and inhibition.
One protein is tagged with NanoLuc luciferase as the energy donor, while the interacting protein is fused to HaloTag as the acceptor. When the proteins interact, the HaloTag ligand binds to HaloTag and comes into close proximity with NanoLuc, producing a detectable BRET signal.
PPI Induction A promoting molecule brings the two proteins closer together. NanoLuc-HaloTag proximity increases and the BRET signal becomes stronger. | PPI Inhibition A disrupting molecule increases the distance between the two proteins. NanoLuc-HaloTag proximity decreases and the BRET signal becomes weaker. |
The assay can therefore capture both positive and negative modulation of protein interactions, making it suitable for studying a broad range of PPI dynamics.
| Full-Length Protein Interactions | Study PPIs involving full-length proteins in their native conformations within live cells. |
| High Sensitivity | The NanoLuc-HaloTag system provides high sensitivity with low background, supporting detection of weak or transient interactions. |
| High-Throughput Compatibility | The assay is compatible with 96- and 384-well plates, supporting large-scale screening and drug-discovery studies. |
Study PPIs in live cells using full-length proteins to better reflect native biological conditions.
Capture both PPI induction and inhibition to study diverse interaction types and compound effects.
Support high-throughput screening of PPI modulators to advance efficient drug discovery.
We value your inquiries and are here to provide you with tailored solutions for your drug discovery and development needs. Whether you have questions, require more information, or are interested in discussing potential collaborations, our team of experts is just a message away.
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