Histone deacetylases (HDACs)

Histone deacetylases (HDACs) are critical enzymes involved in gene-expression regulation through the removal of acetyl groups from histone proteins. This deacetylation process contributes to chromatin remodeling and regulates cellular processes including cell-cycle progression, differentiation, and apoptosis. Aberrant HDAC activity is associated with multiple diseases, particularly cancer, making HDACs important targets for therapeutic research.

HDAC Enzyme Activity Assays

Our HDAC enzyme activity assays use Fluorescence Intensity (FI) technology to detect catalytic activity. A fluorescent dye is attached to a peptide containing acetylated lysine, which quenches the initial fluorescence signal.

When HDAC removes the acetyl group, the fluorescent signal is restored. The increase in fluorescence directly reflects HDAC catalytic activity, providing a sensitive and quantitative readout for inhibitor evaluation.

HDAC enzyme activity assay principle

Quality Control and System Validation

FDA-approved drugs are incorporated as quality-control molecules to verify assay stability, sensitivity, and performance. This validation process supports consistency with published reference data and helps ensure reliable and reproducible results.

Comprehensive HDAC Evaluation Services

01 Subtype Coverage

Testing services are available for common targets across 10 different HDAC subtypes.

02 HDAC Selectivity Profiling

Comparative profiling can be used to evaluate inhibitor selectivity across different HDAC subtypes.

03 Subtype-Specific Evaluation

Focused assays can be configured to assess activity and selectivity against specific HDAC subtypes according to project requirements.

Target Based Assays

Cell Based Assays

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Target Based Assays

Histone deacetylases (HDACs)

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