Cell Adhesion Assay Services

Our Cell Adhesion Assay Services evaluate the adhesion properties of cells, with a focus on integrin–ligand interactions. These interactions play important roles in immune responses, cancer metastasis, tissue repair, and other physiological and pathological processes. The assays provide insights into cell-adhesion mechanisms and their regulation across immunology and general cell-biology research.

Fluorescence Polarization (FP) and ELISA: We offer two biochemical approaches for assessing integrin activity. FP measures the binding affinity of integrins to ligands in solution, providing quantitative information on integrin–ligand interactions. ELISA-based integrin assays measure integrin binding to plate-bound ligands, supporting sensitive and high-throughput analysis.

Integrin–Ligand Binding: Cells expressing integrins are seeded onto plates coated with specific ligands. After a defined binding period, unbound cells are washed away, leaving cells that have adhered through integrin-mediated interactions.

Quantification via CellTiter-Glo: The number of adherent cells is quantified using the CellTiter-Glo (CTG) luminescence assay, which measures cellular ATP as an indicator of viable, adherent cells. This provides a sensitive and accurate readout for evaluating how different factors or treatments affect integrin-mediated adhesion.

The cell adhesion inhibition assay measures the ability of compounds to block the binding of integrins on T cells to their ligands, thereby preventing cell adhesion. Integrins are transmembrane proteins that mediate attachment to the extracellular matrix (ECM) or to other cells. In immune-cell models such as Jurkat T cells and H9 lymphoblast cells, integrins including α4β1, α4β7, and αLβ2 contribute to migration, homing, and tissue infiltration. Blocking these integrins can inhibit immune-cell responses, supporting research into autoimmune diseases, inflammatory conditions, and unwanted immune-cell migration.