In Vitro B Cell Assays

In vitro B-cell assays are important for studying B-cell activation, proliferation and differentiation. They support detailed analysis of antibody production and B-cell responses to antigens, which is relevant to vaccine development and immune-memory research. These assays also help evaluate therapeutic effects on B-cell function, investigate autoimmune disease mechanisms and identify biomarkers for personalized treatment strategies.

B Cell Activation and Proliferation Assays

As B cells proliferate, CFSE is diluted among daughter cells, allowing proliferation rates to be quantified by flow cytometry. CD69 is an early activation marker on lymphocytes, including B cells, and its expression increases shortly after activation.

Plasmablast Antibody Production Assays

This targeted assay supports research into autoimmune diseases such as lupus, rheumatoid arthritis (RA) and multiple sclerosis (MS) by evaluating production of the immunoglobulins IgG and IgM.

Antibody Class Switching Assays

We evaluate the process by which B cells change the class, or isotype, of antibody they produce. The assay typically measures the switch from IgM, the first antibody isotype expressed by naïve B cells, to IgG, a more specialized and abundant isotype produced during immune responses to pathogens.

Antigen Presentation

Our service involves co-culturing B cells with T cells to assess T-cell proliferation and activation. This approach evaluates the ability of B cells to present antigens and stimulate T cells, providing insights into antigen-specific immune responses.

Our comprehensive B-cell assay services are designed to provide reliable and precise data for immunological research. Partner with ICE Bioscience to support studies of B-cell function, therapeutic response and disease biology.