The manual patch clamp (MPC) technique has a storied history as a fundamental tool for electrophysiological research since its development in the late 1970s. It is widely used for its unmatched sensitivity and precision, allowing detailed investigation of ion channel behaviors in various cell types. The manual nature of this method provides a level of control and observation that automated patch clamps can't match, particularly when studying complex or rare ion channel pharmacology. This hands-on approach yields high-quality, reproducible data, essential for in-depth functional analyses in drug development and neurophysiological research. Our service harnesses the power of this technique with unmatched expertise, a broad array of targets, and rapid, cost-effective delivery of results.
✅ Proven Expertise: With over 14 years of specialized experience, we've successfully executed thousands of manual patch clamp projects annually.
✅ Extensive Target Range: Our repertoire includes more than 70 ion channel targets, ensuring comprehensive coverage for diverse research needs.
✅ Speed and Value: We guarantee rapid turnaround, delivering precise manual patch clamp data within only 2 weeks, all at an exceptional cost-to-value ratio.
MPC Assays at Room Temperature and Physiological Temperature
In adherence to the updated ICH S7B Q&A requirements, we've expanded our services to include manual patch clamping at physiological temperatures, significantly enhancing the relevance of our data to human cardiac physiology. This approach provides a more accurate prediction of a drug's cardiac safety profile, mirroring in vivo conditions and ensuring the high fidelity of our cardiac ion channel assays. Our physiologically-temperatured assays represent a significant advancement in predicting clinical outcomes.
Comparison of hERG IC50 values under physiological and room temperature conditions. E4031 and terfenadine exhibit different inhibitory effects on hERG channels at physiological temperature and room temperature. Cisapride exhibits similar inhibitory effects on hERG channels at physiological temperature and room temperature.
Concentration-response curves and current recordings for the effects of Lidocaine and Nifedipine on Nav1.5 and Cav1.2 channels under physiological temperature conditions.
Additional Resources
CASE STUDY:
VX-548's Potent and Selective Inhibition of Nav1.8 Validated by In Vitro Manual Patch-Clamp Analysis
APPLICATION NOTE:
Kv1.3 Inhibition: A Promising Avenue in Immunology and Autoimmune Disease Treatment
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