The cell cycle is the fundamental process through which cells grow, replicate their DNA, and divide. Abnormal cell-cycle regulation is closely linked to diseases including cancer. Cell Cycle Assays analyze the distribution of cells across the G1, S, G2, and M phases, helping researchers evaluate how drugs or genetic modifications affect cell proliferation and growth.
Cells are stained with DNA-binding dyes such as propidium iodide (PI) or DAPI. Because DNA content varies according to cell-cycle phase, flow cytometry can detect and quantify fluorescence intensity as a measure of cellular DNA content.
The resulting data determine the percentage of cells in each cell-cycle phase. This analysis can indicate normal proliferation, arrest in a specific phase, or the presence of a sub-G1 population associated with apoptosis.
Figure. Analysis of cell-cycle distribution in cells treated with PF-06873600, a CDK2/4/6 inhibitor, and DS-8201, a HER2-targeted antibody–drug conjugate (ADC). The histograms show the distribution of cells across the G1, S, and G2 phases after treatment. The percentage of cells in each phase (%G1, %S, and %G2) is provided together with Root Mean Square Deviation (RMSD) and Coefficient of Variation (CV) values for the G1 and G2 phases. The bar charts further illustrate the proportion of cells in each phase for comparative analysis of treatment effects on cell-cycle progression.
With advanced technology and experienced scientific support, ICE Bioscience provides reliable cell-cycle analysis for cancer biology, drug-candidate evaluation, and basic research. The service delivers quantitative information to support interpretation of treatment-related effects on cell proliferation and cell-cycle progression.
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