Generating a high-quality monoclonal stable cell line with stable genetic modifications is essential for studying targets in drug discovery, whether they are GPCRs, ion channels, or kinases, as variable gene expression can lead to non-repeatable results. Genome-editing techniques and interference methodologies are usually adopted to generate protein-overexpressing cell lines, gene mutation cell lines, gene knock-out cell lines, as well as gene knockdown cell lines.
ICE Bioscience utilizes plasmids, lentivirus, and CRISPR approaches to establish a comprehensive cell engineering platform. We have a diverse range of host cells to ensure appropriate overexpression or repressed expression of your target gene. High-content cell imaging systems and fluorescence-activated cell sorting (FACS) are used to improve screening efficiency. Our state-of-the-art cell line development solutions are designed to accelerate customers' drug screening research.
▪ Plasmid-mediated Overexpression: We offer both constitutive and inducible expression systems to suit your project needs.
▪ Lentivirus-mediated Overexpression: Our lentiviral systems are highly efficient, integrating genes into non-dividing cells for stable gene expression.
▪ CRISPR-mediated Gene Editing: From knock-ins and knock-outs to specific point mutations, our CRISPR/Cas9 services provide precise genomic modifications.
▪ High-Content Cell Imaging and FACS: For enhanced screening efficiency, we employ high-content imaging systems and fluorescence-activated cell sorting.
▪ GeCKO v2.0 Library Screening: Our Genome-scale CRISPR Knock-Out library targets a vast array of genes and miRNAs, supported by custom sgRNA library design for tailored research.
▪ Drug target identification
▪ Anti-cancer drug discovery
▪ In vitro tumorigenicity studies
▪ Functional genomics studies
▪ Expertise: Our team’s expertise ensures that your cell lines exhibit the desired gene overexpression or repression, tailored specifically to your target gene.
▪ Technology: Using a high-content cell imaging system and FACS, we maximize screening efficiency and precision.
▪ Innovation: ICE Bioscience remains at the forefront of cell engineering, employing a human genome-scale CRISPR library and customized sgRNA designs for comprehensive gene targeting.
Partner with ICE Bioscience for Cell Engineering Excellence
Our integrated platform, spanning from gene delivery to genomic modification, sets the stage for cutting-edge research and transformative discoveries in drug development. Reach out to ICE Bioscience to advance your research with our bespoke cell engineering solutions.
We value your inquiries and are here to provide you with tailored solutions for your drug discovery and development needs. Whether you have questions, require more information, or are interested in discussing potential collaborations, our team of experts is just a message away.
Feel free to reach out to us.
