GPCR Assay Services

1. 190+ GPCR targets covered across human and preclinical species (including species variants).

2. Various binding and functional assays available.

3. Assessment of agonist, antagonist, and PAM functional modes.

4. Custom cell line develpment services, providing flexibility in cell line choices.

5. Options for single-dose screening or dose-response for EC50 and IC50 determination.

6. Rapid screening with turnaround time of 1-2 weeks.

✅ Biophysical Binding Assay: Our assay combines Biacore 8K's real-time SPR for detailed GPCR-ligand kinetics with Dianthus's Spectral Shift and TRIC technologies for rapid, high-throughput binding assays, providing comprehensive, high-sensitivity binding data.

✅ Radioligand Binding Assay: This assay provides highly sensitive and precise measurements of ligand-receptor interactions.

✅ Flow Cytometry-Based Binding Assay: This assay is valuable for studying cell surface GPCRs, where fluorescently labeled ligands or antibodies can detect and quantify receptor binding in real time.

✅ Tag-Lite Binding Assay: This system operates via a competitive binding format, and the resulting signal change enables quantification of binding affinity.

✅ cAMP Assay: Our GPCR Cell-Based cAMP Assay is specifically designed to measure cAMP levels, providing a key readout for G protein-dependent signaling through Gs or Gi-coupled GPCRs.

✅ IP1 Assay: This assay is designed to measure the buildup of inositol monophosphate (IP1), it's especially valuable for studying GPCRs that activate through the Gq pathway.

✅ Calcium Flux Assay by FLIPR: Our Calcium Flux Assay utilizes the FLIPR (Fluorescence Imaging Plate Reader) system to measure changes in intracellular calcium levels, a key indicator of Gq protein-dependent signaling.

✅ Reporter Gene Assay: The assay measures GPCR activation by utilizing reporter genes like luciferase that are linked to specific signaling pathways.

✅ β-Arrestin Recruitment Assay: Unlike traditional functional assays that measure second messengers (e.g., cAMP or calcium flux), this assay provides insights into β-arrestin-dependent signaling.

✅ GTPγS Binding Assay: GTPγS, a non-hydrolyzable analog of GTP, binds to the G-protein, allowing for the direct assessment of GPCR-mediated G-protein activation without further signaling turnover.

✅ Internalization Assay: High-content imaging allows for real-time, quantitative measurement of receptor internalization following ligand binding.

✅ pERK/pAKT In-Cell Western Assay 

✅ Chemotaxis Assay