ADC In Vitro Biology Study & Screening

Our live-cell imaging-based cytotoxicity assay service provides real-time kinetic analysis of cell viability and cytotoxic effects of therapeutic agents, including ADCs.

Using the Incucyte live-cell imaging system, we provide a dynamic view of ADC effects on cancer-cell populations over time.

ADC Cancer Cell Panels evaluate target-specific ADC efficacy using cancer cell lines from different tumor types and with varying target-expression levels.

Drug-resistant cancer cell models are also available to support ADC resistance profiling and payload-mechanism studies.

Our in vitro bystander-effect assay service evaluates payload diffusion and secondary cytotoxic effects beyond target-positive cells. Using co-cultures with defined target-positive and target-negative cancer-cell populations, we assess payload-mediated bystander killing under controlled conditions to support payload selection, linker optimization, and mechanism-of-action studies.

We provide ADC conjugation and characterization services including size-exclusion chromatography (SEC) for ADC size and aggregation-state analysis, hydrophobic interaction chromatography (HIC) for hydrophobicity and conjugation status, and free payload/linker-payload detection for identifying and quantifying unbound payloads.

DAR analysis quantifies the average number of drug molecules conjugated to each antibody molecule and supports optimization of ADC efficacy and safety.

This service precisely measures target-antigen levels on cell surfaces to support ADC target identification and validation.

Using flow cytometry, we quantify antigen density across cell types to assess target suitability and support selection of high-value targets for next-generation cancer therapies.

SPR provides detailed analysis of binding interactions between therapeutic antibodies and target antigens, supporting effective ADC design. ADC affinity should be similar to that of the corresponding naked antibody.

Flow cytometry analyzes antibody binding to target antigens on the surface of different cell lines.

The service evaluates binding affinity and specificity for antibodies and ADCs across cell lines, supporting targeted delivery and minimizing off-target effects.

Our internalization assay service uses Incucyte live-cell imaging, flow cytometry, and high-content analysis to provide quantitative insight into uptake and intracellular trafficking of fluorescently labelled ADCs in target cancer cells.