In the forefront of modern therapeutic innovation, Targeted Protein Degradation (TPD) represents a groundbreaking approach that is reshaping the landscape of drug discovery. Unlike traditional methods that inhibit protein function, TPD leverages the body's natural cell machinery to selectively degrade and eliminate disease-causing proteins. This revolutionary technique offers a new horizon for tackling previously undruggable targets, opening vast opportunities for the development of novel treatments for a range of intractable diseases.
At ICE Bioscience, our TPD Services are designed to empower your drug discovery journey, offering comprehensive solutions that span from target identification to the development of bespoke degraders. Leveraging cutting-edge technology, deep scientific expertise, and a collaborative approach, we are committed to advancing your projects with precision and efficiency. Whether it’s cancer, neurodegenerative disorders, or other complex conditions, our TPD platform is equipped to meet the challenges of modern medicine, driving forward the discovery of transformative therapeutic agents.
| Category | Solutions | Assays |
|---|---|---|
| In vitro Biology | Protein production | Target protein, E3 |
| Target degradation validation | E3 OE/KD (siRNA, shRNA, sgRNA) | |
| Target KD/KO validation | ||
| HiBiT-fusion protein degradation | ||
| NanoLuc-fusion protein degradation | ||
| Protein t1/2 (CHX inhibition) | ||
| Warhead optimization & screening | E3 binder screening | |
| POI binder screening | ||
| Ternary complex formation assay | ||
| HTRF, AlphaLISA, SPR, MST binding assay | ||
| Cellular degradation and functional assay | WB/JESS | |
| HiBiT-KI cell line construction & HiBiT screening | ||
| In-Cell Western/Immunofluorescence Assay/HTRF/AlphaLISA/MSD/Flow Cytometry/ELISA | ||
| Quantitative Proteomics & Proximity Proteomics | ||
| Cell viability (2D/3D): Cell proliferation, Clonogenic assay | ||
| Downstream signaling protein detection | ||
| Immune functional assays | ||
| Reporter gene assays | ||
| Cell cycle and cell apoptosis assays | ||
| Cell panel screening | ||
| Mechanism & permeability study | Degradation kinetics | |
| Degradation pathway: Proteosome or lysosome | ||
| Proximity-based cellular assay: NanoBRET, NanoBiT, TR-FRET, Co-IP | ||
| Ubiquitination assay | ||
| Degradation selectivity and off-target | ||
| NanoBRET target engagement assay (Live & permeabilized) |
Download our TPD brochure to find out more...
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