Introduction to Reporter Gene Assays

At ICE Bioscience, we specialize in providing state-of-the-art reporter gene assay services. Reporter gene assays are powerful tools used in molecular biology to study gene expression and regulation. By linking a reporter gene to a regulatory sequence of a gene of interest, we can measure the reporter gene’s activity, which reflects the activity of the target gene. This technique is invaluable in drug discovery, functional genomics, and cellular biology.

Our Capabilities

Versatile Cell Line Selection: We offer a diverse array of cell lines, including HEK293, Hela, and more, suitable for both stable and transient transfections in various reporter gene assays.

Target-Specific Assays: Our assays can target specific signaling pathways such as AP-1, NF-κB, CRE, and ARE, utilizing cell lines engineered with precise reporter constructs.

GPCR Signaling Studies: With cell lines like HEK293-GCGR-CRE-Luc2P, we assess GPCR activity, aiding in the discovery of drugs for metabolic disorders and beyond.

Cytokine and Immune Response Profiling: Utilizing HEK-Blue™ cell lines, we measure cytokine activity and immune responses, contributing to immunotherapy and anti-inflammatory drug development.

Nuclear Receptor Research: We study nuclear hormone receptors using cell lines like HEK293-AR-MMTV-Luc2P, providing insights into hormone-related diseases.

DNA Damage Response (DDR) Assays: Our DDR assays, including HR, NHEJ, and TMEJ, are crucial for understanding cancer biology and developing oncology therapeutics.

High-Throughput Screening (HTS): We support HTS for rapid identification of therapeutic compounds.

Custom Assay Development: We develop customized reporter gene assays tailored to specific research needs.

Applications of Reporter Gene Assays

Drug Discovery: Our assays facilitate the identification and validation of new drug targets.

Gene Regulation Studies: We help elucidate the mechanisms of gene regulation in response to various stimuli.

Cellular Pathway Analysis: Our services provide insights into complex cellular signaling pathways.

Safety and Toxicity Testing: We assess the cytotoxicity of compounds, ensuring safety in drug development.

Biological Research: Our assays aid in fundamental biological and disease-oriented research.

At ICE Bioscience, we are committed to advancing scientific discovery by providing reliable, high-quality reporter gene assay services. Contact us to learn how we can support your research and development needs.