ICE Bioscience Posters

Download

G protein-coupled receptors (GPCRS) are the largest family of membrane proteins of cell surface receptors. It can be activated by various stimuli and play an important role in various physiological and pathological processes. Abnormal regulation of GPCR is associated with various human diseases, such as oncology, metabolic diseases, cardiovascular diseases, and eye diseases. GPCR panel has high availability. Here, we constructed over 170 GPCR overexpression stable cell lines, covering wide families like 5-Hydroxytryptamine, acetylcholine, dopamine, glucagon, and opioid receptors. We also constructed different function assays, including cAMP assay, calcium flux assay, IP1 assay, reporter assay, and β-arrestin NanoBit assay to detect the different second messengers produced by G protein submits and GPCR-mediated multiple signaling pathways. The SPR binding assay, tag lite binding assay, and FACS binding assay are constructed to validate the affinity between GPCR and compounds. These GPCR panel and function assays can be used for target identification, hit-to-lead and lead optimization, also suitable for high-throughput screening.