ICE Bioscience Posters

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Methyltransferase-like 3 (METTL3) is the only catalytic subunit of the transferase complex responsible for m6A modification, and it has been found to play a key role in tumorigenesis, tumor growth, metastasis and tumor drug resistance. In recent years, METTL3 has attracted more and more attention as a potential target for cancer treatment.

On this basis, we established an in vitro and in vivo screening platform on METTL3/14 complex, which can realize high-throughput screening of METTL3 inhibitors in vitro experiments. High purity METTL3/14 protein complex has been successfully purified and used in develop METTL3/14 biochemical experiments. We have also established different cellular assays, including proliferation and m6A RNA Methylation quantification assay. Lastly, CDX models utilizing different Ovarian cell lines have been validated. We aim to aid the discovery of new drugs through the METTL3/14 screening cascade.