As one of the important abnormal cytoplasmic DNA monitoring mechanisms, cGAS-STING signaling pathway plays a unique and critical role in host defense against pathogens, tumor immunity, autoimmune diseases, and aging-related inflammation. Sustained activation of the cGAS- STING signaling pathway drives diseases such as autoimmune diseases, aging-associated inflammation, and neurodegenerative pathologies. Therefore, designing drugs that activate/inhibit cGAS or STING signaling may be helpful in anti-cancer, anti-pathogen, anti-inflammatory and other fields. On this basis, we established a series of in vitro screening platforms related to cGAS-STING signaling pathways to facilitate the rapid identification of cGAS/STING agonists/inhibitors.
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