ICE Bioscience Posters

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KRAS molecular glues inhibit KRAS activity by binding to cellular chaperone proteins (such as cyclophilin A, CYPA) to form a ternary complex. The formation of this ternary complex not only blocks the RAS/RAF signaling pathway but also blocks the RAS/SOS1 and RAS/PI3K signaling pathways, thus demonstrating superior inhibitory activity compared to small-molecule inhibitors. Our integrated platform employs advanced in vitro assays to explore these KRAS molecular glues, including KRAS/CYPA ternary complex assay, KRAS(ON)/CYPA/cRAF binding assay, 2D/3D cell proliferation assay, ERK phosphorylation detection, and cellular NanoBRET assay for RAS-RAF disruption. It also includes a p-ERK panel and a 2D/3D cell panel. Overall, we provide a comprehensive platform for the screening and evaluation of KRAS molecular glues, offering a scientific basis for the clinical application of KRAS molecular glues.