Our 3D assay services provide physiologically relevant models for drug discovery by capturing tumor-like architecture, cell–cell interactions and cell–matrix interactions beyond conventional 2D culture.
| Parameter | 2D Cell-based Assay | 3D Cell-based Assay – Spheroids |
|---|---|---|
| Assay format | 96- or 384-well TC-treated flat-bottom plate | 96- or 384-well ultra-low-attachment round-bottom plate |
| Test reagent | 2D CellTiter-Glo | 3D CellTiter-Glo |
| Suitable cell lines | All adherent and suspension cell lines | Most adherent and suspension cell lines |
| Culture duration | Long-term culture | Short-term culture |
| Throughput | High-throughput | Medium-throughput |
| Characteristic | Limited cell–cell and cell–ECM interactions; easy setup and analysis. | More physiologically relevant context that can reflect tumor heterogeneity. |
| Microplate reader | PHERAstar FSX | PHERAstar FSX |
2D assays offer simplicity and affordability. 3D assays provide a more physiologically relevant environment, typically with lower throughput and higher cost.
5–10 day studies in 96- or 384-well ultra-low-attachment plates.
Spheroid formation supports more realistic cell–cell and cell–matrix interactions for tumor-growth and drug-efficacy studies.
3–14 day studies in 96-well plates.
Matrigel embedding supports investigation of invasion, migration, differentiation and drug response in a 3D matrix context.
3–14 day studies in 96-well plates.
Semi-solid agar identifies cells capable of anchorage-independent colony formation, supporting tumorigenicity and transformation studies.
We value your inquiries and are here to provide you with tailored solutions for your drug discovery and development needs. Whether you have questions, require more information, or are interested in discussing potential collaborations, our team of experts is just a message away.
Feel free to reach out to us.
Address: Bldg 16, Yd 18, Kechuang 13th St, Etown, Tongzhou Dist, Beijing, 100176, China
Email: marketing@ice-biosci.com
Tel：+86-10-67809840
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