Deubiquitinating Enzymes (DUBs)

Deubiquitinating enzymes (DUBs) are critical components of the ubiquitin–proteasome system and regulate protein stability, localization, and activity by removing ubiquitin from target proteins. Dysregulation of DUB activity is implicated in cancer, neurodegenerative disorders, and autoimmune conditions. Selective DUB inhibitors can modulate protein degradation pathways and support the discovery of therapeutic strategies for these diseases.

DUB enzymatic activity assays use fluorescence intensity (FI) measurements with Rhodamine 110. In this format, Rhodamine 110 is conjugated to ubiquitin. When a DUB cleaves the ubiquitin–Rhodamine 110 conjugate, fluorescent Rhodamine 110 is released. The resulting increase in fluorescence intensity is measured using a high-sensitivity microplate reader and reflects DUB enzymatic activity.

Whether you require evaluation of an individual DUB or a comprehensive target panel, our deubiquitination assay services provide the flexibility and precision needed to assess the activity and inhibitory potential of DUB-directed compounds.