Part 1 · Target Selection and Validation

Electrophysiological assessment based on ion channels

1. Voltage-gated Na⁺ channels

2. Voltage-gated Ca²⁺ channels

3. Voltage-gated K⁺ channels

4. GABA_A receptors

5. Ionotropic glutamate receptors

Part 2 · In Vitro Pharmacological Evaluation

Cellular and tissue preparations

1. Primary hippocampal neurons

2. Acute brain slices

Induction paradigms

1. Magnesium-free treatment

2. Kainic acid (KA) treatment

3. Glutamate treatment

4. Bicuculline treatment

5. High-potassium treatment

Part 3 · In Vivo Pharmacological Evaluation

In vivo seizure models

1. Maximal electroshock (MES) model

2. 6 Hz psychomotor seizure model

3. PTZ models (i.p. / i.v. / s.c.)

4. Amygdala kindling model

5. Acute febrile seizure model

6. Li-pilocarpine models (acute & chronic)

7. Kainic acid (KA) models (acute & chronic)

Detection methods

1. Behavioral assessment

2. EEG monitoring and analysis

Platform Features

1. Integrated workflow from ion channels to in vivo models

2. Customizable study design

3. Quantitative electrophysiological and behavioral endpoints

4. Standardized procedures supporting reproducibility